The effect of bioidentical nanostructured progesterone in the in vitro culture of preantral follicles and oocyte maturation.
作者： Carlos Cordeiro Neto , Kadja Lopes Soares , Rodrigo Tenório Padilha , Marco Antônio Botelho , Dinalva Brito Queiroz
DOI: 10.1007/S00441-020-03233-6
关键词:
摘要: This study evaluated the effects of bioidentical nanostructured progesterone alone or in association with human chorionic gonadotropin (hCG) on vitro survival and development preantral follicles (experiment 1) oocyte maturation 2). Bioidentical hormones have a molecular structure identical that endogenous hormones; substances refer to those reduced nanoscale. In experiment 1, fragments goat ovarian tissue were cultured for 7 days α-MEM+ supplemented nanoprogesterone (MEM+ + P4) P4 hCG hCG). 2, two mediums (IVM) compared. Medium 1 consisted TCM 199+ LH, medium 2 hCG. The MEM+ treatment showed lowest percentage follicular after culture. treatments higher activation than MEM+. there no differences between all endpoints evaluated. conclusion, addition is advisable culture maturation. However, causes cellular death initial but shows efficacy IVM.
springer.com
sci-hub.st 参考文章(31)
A. MAKRIGIANNAKIS, G. COUKOS, M. CHRISTOFIDOU-SOLOMIDOU, S. MONTAS, C. COUTIFARIS, Progesterone is an autocrine/paracrine regulator of human granulosa cell survival in vitro. Annals of the New York Academy of Sciences. ,vol. 900, pp. 16- 25 ,(2006) , 10.1111/J.1749-6632.2000.TB06212.X
R. N. Chaves, F. S. Martins, M. V. A. Saraiva, J. J. H. Celestino, C. A. P. Lopes, J. C. Correia, I. B. Lima Verde, M. H. T. Matos, S. N. Báo, K. P. O. Name, C. C. Campello, J. R. V. Silva, J. R. Figueiredo, Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro. Reproduction, Fertility and Development. ,vol. 20, pp. 640- 647 ,(2008) , 10.1071/RD07195
Sebastião Freitas de Medeiros, Robert John Norman, None, Formas moleculares da gonadotrofina coriônica humana: características, ensaios e uso clínico Revista Brasileira de Ginecologia e Obstetrícia. ,vol. 28, pp. 251- 263 ,(2006) , 10.1590/S0100-72032006000400008
S. C. J. Hulshof, J. R. Figueiredo, J. F Beckers, M. M. Bevers, R. van den Hurk, Isolation and characterization of preantral follicles from foetal bovine ovaries Veterinary Quarterly. ,vol. 16, pp. 78- 80 ,(1994) , 10.1080/01652176.1994.9694423
Juliana J.H. Celestino, Jamily B. Bruno, Isabel B. Lima-Verde, Maria Helena T. Matos, Mércia Viviane A. Saraiva, Roberta N. Chaves, Fabricio S. Martins, Anderson P. Almeida, Rodrigo M.S. Cunha, Laritza F. Lima, Khesller P.O. Name, Claudio C. Campello, José Roberto V. Silva, Sônia N. Báo, José Ricardo Figueiredo, Steady-state level of kit ligand mRNA in goat ovaries and the role of kit ligand in preantral follicle survival and growth in vitro. Molecular Reproduction and Development. ,vol. 77, pp. 231- 240 ,(2010) , 10.1002/MRD.21138
Frantiescoli A. Dimer, Rossana B. Friedrich, Ruy C. R. Beck, Sílvia S. Guterres, Adriana R. Pohlmann, Impactos da nanotecnologia na saúde: produção de medicamentos Química Nova. ,vol. 36, pp. 1520- 1526 ,(2013) , 10.1590/S0100-40422013001000007
Samira Yarak, Ediléia Bagatin, Karime Marques Hassun, Meire Odete Américo Brasil Parada, Sérgio Talarico Filho, Hiperandrogenismo e pele: síndrome do ovário policístico e resistência periférica à insulina Anais Brasileiros De Dermatologia. ,vol. 80, pp. 395- 410 ,(2005) , 10.1590/S0365-05962005000400011
John J. Peluso, Multiplicity of Progesterone's Actions and Receptors in the Mammalian Ovary Biology of Reproduction. ,vol. 75, pp. 2- 8 ,(2006) , 10.1095/BIOLREPROD.105.049924
MA Botelho, DB Queiroz, G Barros, S Guerreiro, P Fechine, S Umbelino, A Lyra, B Borges, A Freitas, DC Queiroz, R Ruela, JG Almeida, L Quintans Jr, Nanostructured transdermal hormone replacement therapy for relieving menopausal symptoms: a confocal Raman spectroscopy study Clinics. ,vol. 69, pp. 75- 82 ,(2014) , 10.6061/CLINICS/2014(02)01
Kent Holtorf, The Bioidentical Hormone Debate: Are Bioidentical Hormones (Estradiol, Estriol, and Progesterone) Safer or More Efficacious than Commonly Used Synthetic Versions in Hormone Replacement Therapy? Postgraduate Medicine. ,vol. 121, pp. 73- 85 ,(2009) , 10.3810/PGM.2009.01.1949