Enhanced Solvent-Stable Alpha Glycosidase Production by Bacillus licheniformis JXC-1 by Optimization of Feeding Strategies
作者： Jun Fang , Qunfang Tang , Long Liu , Jianghua Li
DOI: 10.1007/978-3-662-46318-5_34
关键词:
摘要: In the previous work, we screened a strain (Bacillus licheniformis JXC-1) producing solvent-stable (10 % N,N-Dimethylformamide, short for DMF) α-glucosidase from several different soil samples. this attempt to improve production by optimization of feeding strategies in 3-L fermenter. Specifically, key factors solvent-tolerant α-glycosidase were investigated first, and optimal conditions (pH 7.0, initial maltose concentration 25 g/L, agitation speed 600 rpm) obtained; enzyme activity reached 444.7 U/L under conditions. Then, four with rates 4–8 h feed or both tryptone carried out, it was found that at rate 2.25 mL/h (4–5 h), 6.75 (5–6 9 (6–7 15 (7–8 h) significantly increased production, 872.5 U/L.
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