Isolation of the V‐ATPase A and c subunit cDNAs from mosquito midgut and malpighian tubules
作者： Sarjeet S. Gill , Phillip B. Chu , Peter Smethurst , Patricia V. Pietrantonio , Linda S. Ross
DOI: 10.1002/(SICI)1520-6327(1998)37:1<80::AID-ARCH10>3.0.CO;2-6
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摘要: Using conserved amino acid sequences for the design of oligonucleotide primers, we isolated cDNA clones two subunits V-ATPase from midgut and Malpighian tubules Aedes aegypti larvae. The 3.1 kb A subunit peripheral catalytic V1 sector codes a protein 68.6 kDa. contains motifs, including an ATP/GTP binding site, found in all other subunits. Southern analysis using as probe suggests presence only single copy gene aegypti. 0.85 c membrane H+ conducting V0 This has four transmembrane domains glutamic that serves site dicyclohexylcarbodiimide. more than one related genome Pileup various shows these fall into distinct clusters, which arthropod proteins are clustered. Arch. Insect Biochem. Physiol. 37:80–90, 1998. © 1998 Wiley-Liss, Inc.
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