HuR cytoplasmic expression is associated with increased cyclin A expression and inferior disease-free survival in patients with gastrointestinal stromal tumours (GISTs).

摘要: Aims HuR is an RNA-binding protein that post-transcriptionally modulates the expression of various target genes involved in carcinogenesis, such as CCNA2, which encodes cyclin A. The aim this study was to evaluate significance HuR and subcellular localization a large cohort gastrointestinal stromal tumours (GISTs). Methods results HuR immunostaining assessable for nuclear cytoplasmic 341 cases on tissue microarrays primary GISTs, 318, 296 193 were also characterized Ki67 labelling, A immunoexpression, KIT PDGFRA receptor tyrosine kinase (RTK) genotypes, respectively. results correlated with disease-free survival (DFS) clinicopathological, immunohistochemical RTK genotypic variables. present 42% significantly related epithelioid histology, larger tumour size, NIH risk category, Importantly, (P < 0.001) overexpression strongly associated worse DFS. Both variables remained independently predictive adverse outcome [P = 0.020 ratio (RR) 2.605 HuR; P = 0.026 RR 2.763 A]. Conclusions HuR not only correlates prognosticators overexpression, but predicts DFS, indicating causative role conferring aggressiveness.