Sialidases of Corynebacteria and their Biotechnological Applications

摘要: Sialidase (EC 3.2.1.18) is a glycosyl hydrolase which hydrolyzes terminal sialic acid residues from the glycans of glycoproteins, glycolipids, and polysaccharides. Corynebacterium diphtheriae harbors an extracellular exo-α-sialidase, NanH, can cleave acids α(2,3)- or α(2,6)-linked to glycoconjugates. These catalytic activities sialidase be used potentially for enzymatical production sialylated complex using regioselective hydrolysis reactions. They also sialylation via transglycosylation. This chapter focuses on biochemical properties structural features C. NanH its homologous proteins synthesize sialyloligosaccharides through chemoenzymatic approaches. In addition, describes potential applications including putative vaccine candidate as virulence factor exoglycosidase analyses glycan structure.