TMC1 and TMC2 Localize at the Site of Mechanotransduction in Mammalian Inner Ear Hair Cell Stereocilia.
作者： Kiyoto Kurima , Seham Ebrahim , Bifeng Pan , Miloslav Sedlacek , Prabuddha Sengupta
DOI: 10.1016/J.CELREP.2015.07.058
关键词:
摘要: Mechanosensitive ion channels at stereocilia tips mediate mechanoelectrical transduction (MET) in inner ear sensory hair cells. Transmembrane channel-like 1 and 2 (TMC1 TMC2) are essential for MET hypothesized to be components of the complex, but evidence their predicted spatiotemporal localization is lacking. Here, we determine TMC proteins mice expressing TMC1-mCherry TMC2-AcGFP. Functionality tagged was verified by transgenic rescue currents hearing Tmc1(Δ/Δ);Tmc2(Δ/Δ) mice. TMC2-AcGFP localize along length immature stereocilia. However, as cells develop, two predominantly tips. Both TMCs absent from tallest stereocilia, where activity not detectable. This distribution confirmed endogenous immunofluorescence. These data consistent with TMC1 TMC2 being channel complex.
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