Bioassay Methods useful to select the natural product cancer chemopreventive agents

摘要: Prevention of malignant disease such as cancer is obviously much more important than therapy, so primary prevention tactics should be invoked with the greatest possible effort. Because delaying a somehow equivalent to cure and it now generally accepted that can managed through chemopreventive interventions. Since considerable number known agents are naturally occuring, reasonable assume additional entities desirable preventive activities exist in nature. In field chemoprevention, definition best bioassay system remains subjective. Use vitro assays permits identification suitable lead starting materials. Assays for monitoring inhibition carcinogenesis at stages initiation (antioxidant activity, antimutagenic induction quinone reductase activity cell culture), promotion (inhibition phorbol ester-induced ornithin decarboxylase culture, cyclooxygenase activity), progression (induction differentiation, anti-estrogenic activity) protein kinase C, established. Unfortunately, animal models or test systems cannot used select useful component due logistical constraints, virtually impossible fully epitomize biological intricacies mammal. Thus, an intermediate solution, mouse gland organ culture model rat tracheal epithelial cells primarily employed secondary evaluation. Prophylactic chemoprevention general population fraught problems, but magnitude potential benefits, imperative continue movement this direction. Several established different stages. The active plant extracts selected procedure. mammal, mammary epitelial evaluation.