Identification of Collagen-Binding Proteins in Lactobacillus spp. with Surface-Enhanced Laser Desorption/Ionization–Time of Flight ProteinChip Technology
作者： Jeffrey C. Howard , Christine Heinemann , Bradley J. Thatcher , Brian Martin , Bing Siang Gan
DOI: 10.1128/AEM.66.10.4396-4400.2000
关键词:
摘要: Biosurfactants produced by Lactobacillus fermentum RC-14, L. rhamnosus GR-1 and 36, casei Shirota were found to contain proteins that bind both collagen types III VI, as determined surface-enhanced laser desorption/ionization (SELDI)–time of flight mass spectrometry. Both VI immobilized on SELDI preactivated ProteinChip arrays detected several different sizes (2 48 kDa) collagen-binding proteins. Overall, the RC-14-produced biosurfactant contained greatest number (RC-14 > 36 Shirota), including mature form a previously cloned 29-kDa protein (referred in its 26-kDa form). Although biosurfactants isolated from also proteins, they do not protein. Together, these results demonstrate utility system means rapidly characterizing clinically important but complex solutions.
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