Advances in Host and Vector Development for the Production of Plasmid DNA Vaccines

摘要: Recent developments in DNA vaccine research provide a new momentum for this rather young and potentially disruptive technology. Gene-based vaccines are capable of eliciting protective immunity humans to persistent intracellular pathogens, such as HIV, malaria, tuberculosis, which the conventional technologies have failed so far. The recent identification characterization genes coding tumor antigens has stimulated development DNA-based antigen-specific cancer vaccines. Although most academic researchers consider production reasonable amounts plasmid (pDNA) immunological studies relatively easy solve, problems often arise during first phase production. In chapter we review current state art pDNA at small (shake flasks) mid-scales (lab-scale bioreactor fermentations) address trends vector design strain engineering. We will guide reader through different stages process starting from choosing appropriate backbone, right Escherichia coli (E. coli) production, cultivation media scale-up issues. addition, some points concerning safety potency produced plasmids, with special focus on producing antibiotic resistance-free plasmids. main goal is make immunologists aware fact that be performed much attention care rest their research.