REGIONAL HETEROGENEITY AND DIVERSITY OF ASTROCYTES IN RESPONSE TO HIV-1 PROTEINS AND MORPHINE

摘要: HIV-infected individuals who abuse opiates have been found to a higher incidence and faster progression of HIV encephalitis. Astrocytes, the major support cells in CNS, are known play critical role neuropathy. Although astrocytes tend not be productively infected by HIV-1 virus, dysregulation their proand antiinflammatory cytokines/chemokines secretion is usually neurotoxic. Glutamate transport reported impaired as well, which result extrasynaptic excitatory neurotransmitter accumulation over stimulation postsynaptic neurons. It long that from different brain regions diverse responses extracellular stimulants. However, few publications discuss regional heterogeneity central nervous system. Based on above information, we hypothesize astroglia vary proteins could influenced co-treatment morphine. To test this cultured cerebral cortex, cerebellum spinal cord, treated them with Tat gp120 or without morphine opioid receptor (mu, delta kappa receptor) antagonist, naloxone. After 12-18 hours, conditioned medium each group was analyzed using Bio-Plex array. Cells striatal cultures were harvested lysed; extracted evaluated Western blotting see whether EAAT2 expression changed. Results showed there significant differences among three