RecA homologous DNA transferase: Functional activities and a search for homology by recombining DNA molecules

摘要: Bacterial RecA is a prototype of ATP-dependent homologous recombinases, found ubiquitously from bacteriophages to humans. The filament formed on single-stranded DNA in the presence ATP initiates strand exchange reaction with double-stranded DNA. Of three stages this (search for homology, annealing triple-stranded structure accompanied by switch pairing, and displacement third strand), first stage most enigmatic least studied. As generally accepted, directed special (extended) does not require any additional energy hydrolysis. new approaches study short oligonucleotides as substrates sensitive methods real-time monitoring suggest that all depend