Eupatolide inhibits PDGF-induced proliferation and migration of aortic smooth muscle cells through ROS-dependent heme oxygenase-1 induction.

摘要: The abnormal proliferation and migration of vascular smooth muscle cell (VSMC) contributes importantly to the pathogenesis atherosclerosis restenosis. Here, we investigated effects eupatolide (EuTL), a sesquiterpene lactone isolated from medicinal plant Inula britannica, on platelet-derived growth factor (PDGF)-induced primary rat aortic cells (RASMCs), as well its underlying mechanisms. EuTL remarkably inhibited PDGF-induced RASMCs. Treatment RASMCs with induced both protein mRNA expression heme oxygenase-1 (HO-1). SB203580 (a p38 inhibitor), SP600125 JNK U0126 MEK inhibitor) LY294002 PI3K did not suppress EuTL-induced HO-1 expression; however, N-acetylcysteine (NAC, an antioxidant) blocked expression. Moreover, treatment increased reactive oxygen species (ROS) accumulation nuclear translocation factor-E2-related 2 (Nrf2); this was also by NAC. NAC or inhibition significantly attenuated inhibitory Taken together, these findings suggest that could VSMCs through induction via ROS-Nrf2 pathway may be potential inducer for preventing treating diseases. Copyright © 2013 John Wiley & Sons, Ltd.