Cloning and expression of five heat shock protein genes in relation to cold hardening and development in the leafminer, Liriomyza sativa.

摘要: The vegetable leafminer, Liriomyza sativae has spread worldwide, causing serious loss of agricultural productivity. Heat shock proteins (HSPs) play important roles in the environmental adaptation various organisms, and to explore functions HSPs relation cold tolerance development L. sativae, three full-length cDNAs small heat protein genes (ls-hsp19.5, ls-hsp20.8 ls-hsp21.7) two partial tcp1 (the hsp60 homolog, ls-tcp1alpha ls-tcp1zeta) were cloned, their transcriptional expression during hardening was examined by real time quantitative PCR. open reading frames (ORFs) ls-hsp19.5, ls-hsp21.7 are 516, 543 573bp length, encoding with molecular weights (M.W.) 19.5, 20.8 21.7kDa, respectively. 956 323bp respectively sequenced from ls-tcp1zeta. profiles revealed that ls-tcp1s did not respond stress. However, hsps significantly induced cold, more cold-sensitive than others. These results suggest different shsp members may be responsible for stresses intensity. developmental processes mRNA levels reached a peak pupal stage, whereas large hsps, including ls-tcp1s, hsp90 increased gradually process. that, addition response, these involved sativae.