An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris).
作者： Zhiqun Che , Xiaoyan Cao , Guiguang Chen , Zhiqun Liang
DOI: 10.1186/S12896-020-00654-7
关键词:
摘要: … After codon optimization, the fibase from a marine Bacillus subtilis was expressed and secreted in K. phaffii GS115. Recombinant strains harboring different copies of the fib gene (fib-nc…
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