Thioredoxin-1/peroxiredoxin-1 as sensors of oxidative stress mediated by NADPH oxidase activity in atherosclerosis

摘要: To assess the potential association between TRX-1/PRX-1 and NADPH oxidase (Nox) activity in vivo vitro, levels were assessed by ELISA 84 asymptomatic subjects with known phagocytic carotid intima-media thickness (IMT). We found a positive correlation oxidase-dependent superoxide production (r=0.48 0.47; p<0.001 for both) IMT (r=0.31 0.36; p<0.01 adjusted age sex. Moreover, plaques have higher PRX-1 TRX plasma (p<0.01 both). These data confirmed second study which patients atherosclerosis showed than healthy (p<0.001 In human atherosclerotic plaques, subunit p22phox colocalized macrophages (immunohistochemistry). monocytes macrophages, phorbol 12-myristate 13-acetate (PMA) induced activation release to extracellular medium, concomitant decrease their intracellular levels, was reversed inhibitor apocynin (Western blot). loss-of-function experiments, genetic silencing of Nox2 blocked PMA-induced downregulation macrophages. Furthermore, involves modulation redox status exosome-like vesicles. are associated oxidase-activity vitro. could suggest coordinated antioxidant response oxidative stress atherothrombosis.