Transcriptomic analysis of the host response to an iridovirus infection in Chinese giant salamander, Andrias davidianus.

摘要: The emergence of an infectious viral disease caused by the Chinese giant salamander iridovirus (GSIV) has led to substantial economic losses. However, no more molecular information is available for understanding mechanisms associated with virus–host interaction. In this study, de novo sequencing was used obtain abundant high-quality ESTs and investigate differentially-expressed genes in spleen salamanders that were either infected or mock GSIV. Comparative expression analysis indicated 293 down-regulated 220 up-regulated. Further enrichment showed most enriched pathway “complement coagulation cascades”, significantly diseases include “inherited thrombophilia”, “immune system diseases”, “primary immunodeficiency”, regulatory protein defects”, “disorders nucleotide excision repair”. Additionally, 30 678 simple sequence repeats (SSRs) from all samples, 26 355 single polymorphisms (SNPs) spleens uninfected animals 36 070 SNPs detected. large amount variation specific results reported herein provided significant new EST could contribute greatly investigations into functions immune salamander.