Adipose differentiation‑related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation.

摘要: Vascular smooth muscle cells (VSMCs) have an important role in atherosclerosis development. Evidence has demonstrated that adipose differentiation‑related protein (ADRP) is associated with foam cell formation and progression. However, to the best of our knowledge, no previous studies investigated ADRP knockdown platelet‑derived growth factor (PDGF)‑stimulated proliferation migration VSMCs vitro. Furthermore, effect on neointima vivo remains unclear. In present study, primary human aortic were incubated PDGF following small interfering (si)RNA transfection. Cell viability, cycle distribution analyzed by MTT, wound healing Transwell assays flow cytometry, respectively. Extracellular signal‑regulated kinase (ERK), phosphorylated (p)‑ERK, Akt, p‑Akt, proliferating nuclear antigen (PCNA), matrix metalloproteinase (MMP)‑2 MMP‑9 levels determined western blotting. Apolipoprotein E-/- mice fed atherogenic diet injected siADRP or control siRNA twice a week. After 3 weeks therapy, aortas excised mRNA expression was determined. Neointima assessed hematoxylin eosin staining. The results current study significantly inhibited PDGF‑induced increases VSMC caused G1 phase arrest decreased PCNA expression. Knockdown reducing MMP activity. addition, also ERK Akt signaling pathways response PDGF. administration suppressed mouse model. indicate may be potential target for treatment atherosclerosis.