cDNA cloning of galectins from third stage larvae of the parasitic nematode Teladorsagia circumcincta.

摘要: Abstract A monoclonal antibody raised to a Teladorsagia circumcincta 31–33 kDa doublet antigen was used immunoscreen T. cDNA expression library. Sheep antibodies eluted from the proteins expressed by two clones immunopositive with specifically recognised on Western blots of third stage larval extract, confirming that these coded for antigen. Database searches revealed high levels similarity β -galactoside-binding lectin-like (GalBPs or galectins) Caenorhabditis elegans and Onchocerca volvulus . By analogy sequences, both contain full-length protein coding region. The native could be preferentially extracted homogenates larvae lactose affinity purified an asialofetuin column, identity bands as galectins. Reverse transcriptase-polymerase chain reaction amplification using primer based C. Spliced Leader SL1 sequence showed corresponding mRNAs are also trans -spliced at their 5′ ends. While there considerable nucleotide differences between clones, majority located in non-coding regions. Within region 87 but only three result amino acid substitutions.