Recent Speciation in Three Closely Related Sympatric Specialists: Inferences Using Multi-Locus Sequence, Post-Mating Isolation and Endosymbiont Data

摘要: Shifting between unrelated host plants is relatively rare for phytophagous insects, and distinct specificity may play crucial roles in reproductive isolation. However, the isolation status relationship parental divergence post-mating among closely related sympatric specialists are still poorly understood. Here, multi-locus sequence were used to estimate three plant–specific flea beetles, Altica cirsicola, A. fragariae viridicyanea (abbreviated as AC, AF AV respectively). The tree topologies inconsistent using different gene or combinations of fragments. AF+(AC+AV) was supported, however, by both species based on concatenated data. Post-mating data results crossing these best interpreted light a well established phylogeny. Nuclear-induced but not Wolbachia-induced unidirectional cytoplasmic incompatibility, which detected AC-AF AF-AV AC-AV, also suggest more close genetic affinity AC AV. Prevalence Wolbachia endosymbiont most individuals sharing same wsp haplotype give another evidence AF+(AC+AV). Our study suggested that beetles diverged relative short time (0.94 My), be result shifting specificity. Incomplete while almost complete lineage sorting indicated effective pre-mating should have evolved.