Gut microbial diversity is reduced and is associated with colonic inflammation in a piglet model of short bowel syndrome
作者： Susan Lapthorne , Prue M. Pereira-Fantini , Fiona Fouhy , Guineva Wilson , Sarah L. Thomas
DOI: 10.4161/GMIC.24372
关键词:
摘要: Background and objectives: Following small bowel resection (SBR), the luminal environment is altered, which contributes to clinical manifestations of short syndrome (SBS) including malabsorption, mucosal inflammation bacterial overgrowth. However, impact SBR on colon has not been well-defined. The aims this study were characterize colonic microbiota following assess in colon.Results Analysis demonstrated that there was a significant level dysbiosis both two six weeks post-SBR, particularly phylum Firmicutes, coupled with decrease overall diversity colon. This associated an increase post-surgery.MethodsFemale (4-week old) piglets (5−6/group) received 75% SBR, transection (sham) or no surgery. Compositional analysis performed by high-throughput sequencing, two- a...
sci-hub.se 参考文章(47)
Ruifrok Ac, Johnston Da, Quantification of histochemical staining by color deconvolution Analytical and Quantitative Cytology and Histology. ,vol. 23, pp. 291- 299 ,(2001)
K H Wilson, R B Blitchington, R C Greene, Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction. Journal of Clinical Microbiology. ,vol. 28, pp. 1942- 1946 ,(1990) , 10.1128/JCM.28.9.1942-1946.1990
Susan Lapthorne, John MacSharry, Paul Scully, Kenneth Nally, Fergus Shanahan, Differential intestinal M-cell gene expression response to gut commensals. Immunology. ,vol. 136, pp. 312- 324 ,(2012) , 10.1111/J.1365-2567.2012.03581.X
Kate L. Healey, Julie E. Bines, Sarah L. Thomas, Guineva Wilson, Russell G. Taylor, Magdy Sourial, Prue M. Pereira-Fantini, Morphological and functional changes in the colon after massive small bowel resection. Journal of Pediatric Surgery. ,vol. 45, pp. 1581- 1590 ,(2010) , 10.1016/J.JPEDSURG.2010.02.040
J Schalamon, J.M Mayr, M.E Höllwarth, Mortality and economics in short bowel syndrome Best Practice & Research Clinical Gastroenterology. ,vol. 17, pp. 931- 942 ,(2003) , 10.1016/S1521-6918(03)00079-9
Camille Mayeur, Jean-Jacques Gratadoux, Chantal Bridonneau, Fatima Chegdani, Béatrice Larroque, Nathalie Kapel, Olivier Corcos, Muriel Thomas, Francisca Joly, Faecal D/L Lactate Ratio Is a Metabolic Signature of Microbiota Imbalance in Patients with Short Bowel Syndrome PLoS ONE. ,vol. 8, pp. e54335- ,(2013) , 10.1371/JOURNAL.PONE.0054335
Conrad R Cole, Nellie I Hansen, Rosemary D Higgins, Thomas R Ziegler, Barbara J Stoll, Eunice Kennedy Shriver NICHD Neonatal Research Network, None, Very low birth weight preterm infants with surgical short bowel syndrome: incidence, morbidity and mortality, and growth outcomes at 18 to 22 months. Pediatrics. ,vol. 122, ,(2008) , 10.1542/PEDS.2007-3449
O. Goulet, F. Joly, Intestinal microbiota in short bowel syndrome Gastroenterologie Clinique Et Biologique. ,vol. 34, ,(2010) , 10.1016/S0399-8320(10)70019-1
Ger P. A. Bongaerts, René S. V. M. Severijnen, Albert Tangerman, Aad Verrips, Jules J. M. Tolboom, Bile acid deconjugation by Lactobacilli and its effects in patients with a short small bowel Journal of Gastroenterology. ,vol. 35, pp. 801- 804 ,(2000) , 10.1007/S005350070016
Melanie Craven, Charlotte E. Egan, Scot E. Dowd, Sean P. McDonough, Belgin Dogan, Eric Y. Denkers, Dwight Bowman, Ellen J. Scherl, Kenneth W. Simpson, Inflammation Drives Dysbiosis and Bacterial Invasion in Murine Models of Ileal Crohn’s Disease PLoS ONE. ,vol. 7, pp. e41594- ,(2012) , 10.1371/JOURNAL.PONE.0041594