Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

摘要: Non-biofouling surfaces constitute one of the most important subjects in sensitively and selectively detecting biomolecular events. Poly(ethylene glycol) (PEG) chains tethered on substrate are well known to reduce non-biofouling characteristics. Protein adsorption onto a PEG-chain-tethered surface is strongly influenced by density PEG chain almost completely suppressed successive treatment longer (5 kDa) followed (2 kDa; mixed-PEG-chain-tethered surface) because significant increase density. To modify versatile surface, possessing pentaethylenehexamine at end (N6-PEG) was prepared via reductive amination reaction aldehyde-ended with pentaethylenehexamine. Using N6-PEG, antibody/PEG co-immobilization conducted active ester groups. After antibody immobilized were constructed surrounding antibody. It interesting note that functions not only as non-fouling agent but also improves immune response. The hybrid applied oligo DNA immobilization. DNA/PEG improved hybridization, retaining its ability. Densely packed antibodies and/or their orientation surface. Thus, this material promising high-performance biointerface for applications. poly(ethylene (PEG)-tethered-chain mixed-PEG tethered-chain pentaethylenehexamine-ended PEG, which newly designed ourselves, oligoDNA/PEG co-immobilizations carried out. found work character an improvement biomolecules. it