Functional Analysis of a Predicted Flavonol Synthase Gene Family in Arabidopsis
作者： Daniel K. Owens , Anne B. Alerding , Kevin C. Crosby , Aloka B. Bandara , James H. Westwood
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摘要: The genome of Arabidopsis (Arabidopsis thaliana) contains five sequences with high similarity to FLAVONOL SYNTHASE1 (AtFLS1), a previously characterized flavonol synthase gene that plays central role in flavonoid metabolism. This apparent redundancy suggests the possibility uses multiple isoforms FLS different substrate specificities mediate production flavonols, quercetin and kaempferol, tissue-specific inducible manner. However, biochemical genetic analysis six AtFLS indicates that, although several members are expressed, only AtFLS1 encodes catalytically competent protein. also appears be member this group influences levels root gravitropic response seedlings under nonstressed conditions. study showed other expressed have tissue- cell type-specific promoter activities overlap those encode proteins interact enzymes yeast two-hybrid assays. Thus, it is possible these "pseudogenes" alternative, noncatalytic functions not yet been uncovered.
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