A novel method for quantifying peripheral tissue amyloid load by using the radiolabeled amyloidophilic peptide, p5

摘要: Quantitation of peripheral amyloid deposits by non-invasive molecular imaging can be useful for diagnosis, prognostication and monitoring response to therapy. In order obtain reliable quantitative data, it is necessary show a linear positive correlation between the uptake probe tissue load. The transgenic H-2/IL-6 mouse model AA amyloidosis was used generate animals with varied stages visceral disease. mice were injected 125I-labeled peptide p5 tissues analyzed 2 h post-injection using Congo red (CR) staining, radioisotope biodistribution micro-autoradiography (ARG). Micro-ARG confirmed that 125I-p5 deposited at all sites Congophilia but not amyloid-free within evaluated. Furthermore, studies revealed amount 125I in liver spleen correlated CR birefringence (expressed as 0-4+ or area [µm2]) these coefficients r > 0.7 (p < 10(-6)). Deposition measure this model. has potential agent human