An In Vitro Model for Tumor Progression in Murine Lymphoid Cells

摘要: Histopathological observations of the development tumors have led to concept that oncogenesis proceeds through sequential acquisition independent growth-related phenotypic characteristics (Foulds 1975). This process tumor progression occurs events involving expression specific genes affecting regulation cellular growth (recently reviewed in Weinberg 1985; Klein and Bishop 1985). The requirement two complementing oncogenes for vitro transformation primary rat embryo firbroblasts provides a useful experimental model progression. Ha-ras (EJ) v-myc or (T24) E1A can act together transform fibroblasts, while neither oncogene acting alone is capable causing efficient (Land et al. 1983; Ruley 1983) unless expressed at high level (Spandidos Wilkie 1984).