Comparative population genomic analyses of transporters within the Asgard archaeal superphylum.

摘要: Upon discovery of the first archaeal species in 1970s, life has been subdivided into three domains: Eukarya, Archaea, and Bacteria. However, organization three-domain tree challenged following lineages such as TACK Asgard superphyla. The Superphylum emerged closest ancestor to eukaryotes, potentially improving our understanding evolution forms. We characterized transportomes their substrates within four metagenome-assembled genomes (MAGs), that is, Odin-, Thor-, Heimdall- Loki-archaeota well fully sequenced genome Candidatus Prometheoarchaeum syntrophicum strain MK-D1 belongs Loki phylum. Using Transporter Classification Database (TCDB) reference, candidate transporters encoded proteomes were identified based on sequence similarity, alignment coverage, compatibility hydropathy profiles, TMS topologies shared domains. Identified transport systems compared superphylum dissimilar eukaryotic, bacterial organisms. From these analyses, we infer organisms rely mostly driven by proton motive force (pmf), electrochemical gradient which then can be used for ATP production drive activities secondary carriers. results indicate archaea depend heavily uptake organic molecules lipid precursors, amino acids derivatives, sugars derivatives. Overall, majority are more similar prokaryotic than eukaryotic although several instances reverse documented. Taken together, support previous suggestions includes largely mixotrophic anaerobic but clearly define metabolic potential while providing evidence regarding relatedness eukaryotes.