The effects of sequential inoculation of mixed rumen protozoa on the degradation of orchard grass cell walls by anaerobic fungus Anaeromyces mucronatus 543.

摘要: The effects of protozoa on the degradation plant cell walls (CW) during different growth stages fungus Anaeromyces mucronatus have been investigated. Since fungi show a marked lag in their vitro cultures and many rapidly die prolonged incubation time, may vary according to phase fungi. Therefore, approach adopted was (i) inoculate CW with monoculture, (ii) fungus-protozoa coculture, or (iii) sequentially fungal that had grown for 24 (initial stage growth), 48, 72 h (late growth) mixed protozoa. When associated protozoa, dependent effect observed. Ruminal adversely affected activity when introduced initial A. mucronatus, but synergetic interaction detected added late cultures. Although there is no immediate explanation these results, ...