Correlation between env V1/V2 Region Diversification and Neutralizing Antibodies during Primary Infection by Simian Immunodeficiency Virus sm in Rhesus Macaques

摘要: Evolution of the domain encoding V1/V2 variable region simian immunodeficiency virus sm (SIVsm) envelope (env) gene was analyzed in relation to route challenge, load, and neutralizing antibody (NAb) titers during primary infection rhesus macaques with pathogenic SIVsmE660 isolate. In this model system animals are initially infected multiple viruses as evidenced by presence genotypic variants that could be resolved using a heteroduplex tracking assay (HTA). Overlapping subsets were established each animal. There no selection for establishment specific comparing intravenous- intrarectal-challenged at week 2 postinfection, suggesting occurred mucosal surface. an initial period significant stability variants. Macaques challenged intravenously displayed subsequent diversification significantly earlier than intrarectally well past resolution viremia. The time when SIVsmE660-specific NAbs reached threshold titer 100 correlated timing diversification, even though antibodies Env protein detected much earlier. 400 load late infection. These results show affects is maturation NAb response. However, prior immunization capable priming anamnestic response did not accelerate diversification. This result suggests SIV env type-specific