An overview of colorimetric assay methods used to assess survival or proliferation of mammalian cells.

摘要: The aim of this review is to briefly describe some colorimetric methods that are commonly used evaluate a new chemical entity (NCE) on cell cultures in non-clinical oncology discovery research. These have the distinct advantage over other techniques they can be applied and monolayer or suspension culture. Both protein assay determination viability assays may conducted using these culture systems. routinely assessed by utilizing metabolic capacity cells which biochemically convert chemicals (usually color dyes) then conveniently measured at specific wavelengths multi-well plate reader. Resazurin (Alamar Blue) an example one metabolically active compounds. nontoxic dye also measure migration cellular invasion without resorting sacrifice during test procedure. Another 5-bromo-2-deoxyuridine (bromodeoxyuridine BrdU) thymidine analog incorporates into DNA dividing S-phase cycle. We will discuss version traditional 3H-thymidine incorporation immunoenzymatic synthesis its application