Fractionation of proteins and carbohydrates from crude microalgae extracts using an ionic liquid based-aqueous two phase system

摘要: Abstract Mild, simple and efficient recovery methods are required to obtain high-value microalgae proteins. As a promising extraction method, an Aqueous two phase system (ATPS) was used partition proteins from crude extracts obtained green of industrial interest: Neochloris oleoabundans Tetraselmis suecica . Furthermore, the Non-Random Two Liquids model (NRTL) applied describe both diagram coefficient total protein. It observed that protein preferentially concentrates in top phase. Additionally, no significant effect on or efficiency noted at different tie lines. Experimental data indicate sugars selectively fractionated bottom phases respectively. The provided good representation experimental for liquid-liquid equilibrium. Moreover, also led partitioning reference proteins, Rubisco Bovine Serum Albumin (BSA), as well extracts.