Analysis of alpha-1-acid glycoprotein isoforms using CE-LIF with fluorescent thiol derivatization
作者： Raúl Garrido-Medina , Angel Puerta , Zuly Rivera-Monroy , Mercedes de Frutos , Andras Guttman
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摘要: The analysis of glycoprotein isoforms is high interest in the biomedical field and clinical chemistry. Many studies have demonstrated that some could serve as biomarkers for several major diseases, such cancers vascular among others. Capillary zone electrophoresis (CZE) a well-established technique to separate isoforms, however, it suffers from limited sensitivity when UV-Vis detection used. On other hand, with laser-induced fluorescence (LIF) detection, derivatization reaction render proteins fluorescent can destroy resolution isoforms. In this work, procedure through thiol groups glycoproteins using either 5-(iodoacetamide) fluorescein (5-IAF) or BODIPY iodoacetamide presented model protein alpha-1-acid (AGP). process enabled high-resolution AGP by CZE-LIF. was successfully applied label samples serum secretome artery tissue, enabling separation CE-LIF natural at different concentration levels.
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