The Effect of Attachment of Hydrophobic Modifiers on the Catalytic Activities of Lipase and Trypsin
作者： K. Ampon , M. Basri , C.N.A. Razak , W.M.Z. Yunus , A.B. Salleh
DOI: 10.1016/B978-0-444-89046-7.50052-7
关键词:
摘要: Abstract Several hydrophobic groups of different sizes and steric hindrance have been covalently attached to lipase trypsin. Attachments polyethylene glycol chemical modification by reductive alkylation amidination with aldehydes imidoesters, respectively, increased the ester synthesis activities these enzymes 2–6 folds compared native unmodified enzymes. The maximal esterification alkylated appeared depend on particular aldehyde degree modification.For example, required a lower more bulky (eg. dodecyldehyde) attain its activity that which was modified acetaldehyde. Excessive modifications result in decrease activity. Trypsins were derivatized their aldehydes, very similar hydrophobicity. Most thus prepared active thermostable anhydrous organic solvents used, particularly dimethyformamide (DMF) pyridine. Modifications imidoesters gave results those obtained aldehydes.
sci-hub.st 参考文章(10)
Vadim V. MOZHAEV, Yuri L. KHMELNITSKY, Mariya V. SERGEEVA, Alla B. BELOVA, Nataliya L. KLYACHKO, Andrey V. LEVASHOV, Karel MARTINEK, Catalytic activity and denaturation of enzymes in water/organic cosolvent mixtures FEBS Journal. ,vol. 184, pp. 597- 602 ,(1989) , 10.1111/J.1432-1033.1989.TB15055.X
V.P. Torchilin, V.G. Omel'Yanenko, A.L. Klibanov, A.I. Mikhailov, V.I. Gol'Danskii, V.N. Smirnov, Incorporation of hydrophilic protein modified with hydrophobic agent into liposome membrane. Biochimica et Biophysica Acta. ,vol. 602, pp. 511- 521 ,(1980) , 10.1016/0005-2736(80)90330-2
Kamaruzaman Ampon, Gary E. Means, Immobilization of proteins on organic polymer beads Biotechnology and Bioengineering. ,vol. 32, pp. 689- 697 ,(1988) , 10.1002/BIT.260320514
K. Ampon, A. B. Salleh, A. Teoh, W. M. Z. Wan Yunus, C. N. A. Razak, M. Basri, SUGAR ESTERIFICATION CATALYSED BY ALKYLATED TRYPSIN IN DIMETHYLFORMAMIDE Biotechnology Letters. ,vol. 13, pp. 25- 30 ,(1991) , 10.1007/BF01033511
F. M. Veronese, R. Largajolli, E. Boccú, C. A. Benassi, O. Schiavon, Surface modification of proteins. Activation of monomethoxy-polyethylene glycols by phenylchloroformates and modification of ribonuclease and superoxide dismutase. Applied Biochemistry and Biotechnology. ,vol. 11, pp. 141- 152 ,(1985) , 10.1007/BF02798546
K. Ampon, A.B. Salleh, F. Salam, W.M.Z. Wan Yunus, C.N.A. Razak, M. Basri, Reductive alkylation of lipase Enzyme and Microbial Technology. ,vol. 13, pp. 597- 601 ,(1991) , 10.1016/0141-0229(91)90097-T
KRISTEN FRETHEIM, SHIGEO IWAI, ROBERT E. FEENEY, EXTENSIVE MODIFICATION OF PROTEIN AMINO GROUPS BY REDUCTIVE ADDITION OF DIFFERENT SIZED SUBSTITUENTS International Journal of Peptide and Protein Research. ,vol. 14, pp. 451- 456 ,(2009) , 10.1111/J.1399-3011.1979.TB01956.X
Yuji Inada, Hiroyuki Nishimura, Katsunobu Takahashi, Takayuki Yoshimoto, Anutosh Ranjan Saha, Yuji Saito, Ester synthesis catalyzed by polyethylene glycol-modified lipase in benzene Biochemical and Biophysical Research Communications. ,vol. 122, pp. 845- 850 ,(1984) , 10.1016/S0006-291X(84)80111-4
R Fields, The measurement of amino groups in proteins and peptides Biochemical Journal. ,vol. 124, pp. 581- 590 ,(1971) , 10.1042/BJ1240581
M. J. Hunter, M. L. Ludwig, The Reaction of Imidoesters with Proteins and Related Small Molecules Journal of the American Chemical Society. ,vol. 84, pp. 3491- 3504 ,(1962) , 10.1021/JA00877A016