Neuro- and immunotoxic effects of fumonisin B1 in cells

摘要: Fumonisin B1 (FB1) is a mycotoxin produced by the fungus Fusarium verticillioides, which commonly infects corn and other agricultural products. species can also be found in moisture-damaged buildings, therefore there may human exposure to mycotoxins, including FB1. FB1 known affect metabolism of sphingolipids inhibiting enzyme ceramide synthase. It neuro-, hepatoand nephrotoxic, it classified as possibly carcinogenic humans. This study aimed clarify mechanisms behind FB1-induced neuroand immunotoxicity. Four neural glial cell lines human, rat mouse origin were exposed graded doses The effects on production reactive oxygen species, lipid peroxidation, intracellular glutathione levels, viability apoptosis investigated at different time points (0.5-144 h). Furthermore, FB1, alone or together with lipopolysaccharide (LPS), mRNA protein expression levels cytokines chemokines studied dendritic cells (DC), differentiated from peripheral blood mononuclear cells. induced oxidative stress death all studied. However, sensitivities varied depending parameter was being Generally, only seen after prolonged 10 100 μM Signs apoptosis, reflected increased caspase-3-like protease activity internucleosomal DNA fragmentation, four lines. results provide evidence that potential neurotoxin vitro, but magnitude toxic line dependent. used this towards U-118MG glioblastoma > GT1-7 hypothalamic C6 SH-SY5Y neuroblastoma When comparing origin, concluded seem more sensitive toxicity than those origin. After for 6 24 h, significantly cytokine interferon-γ (IFNγ) detected DC. observation further confirmed chemokine CXCL9, regulated IFNγ. During co-exposure DC both LPS significant inhibitions LPS-induced pro-inflammatory interleukin-6 (IL-6) IL-1β, their regulatory CCL3 CCL5 observed. show has capacity modulate immune responses Therefore, rather likely affects types participating defence system. evaluating important consider cell-cell interactions. represent new information, especially about stress, immunotoxicity, well varying