Prognostic value of histone chaperone FACT subunits expression in breast cancer.

摘要: Understanding the underlying reasons for tumor aggressiveness, such as why some tumors grow slowly and locally, while others rapidly progress to a lethal metastatic disease, is still limited. This especially critical in breast cancer (BrCa) due its high prevalence also possibility that it can be detected early. Several oncogenes suppressors have been identified are used prognosis treatment of BrCa. However, even with these markers, outcome within BrCa subtypes highly variable. Chromatin organization has long acknowledged factor plays an important role progression, but molecular mechanisms defining chromatin dynamics largely missing. We recently found histone chaperone FACT (facilitates transcription) overexpressed ~18-20% cases. elevated upon transformation mammary epithelial cells essential viability cells. more aggressive transcriptional program than those low Based on this we propose may marker In study, aimed comprehensively characterize pattern expression relation other clinical prognostic markers. developed tested assay detection quantitation protein levels both subunits, SSRP1, SPT16, samples. compared value mRNA potential markers disease aggressiveness using large cohort patients (n=1092). demonstrated only SSRP1 immunohistochemical staining reliable indicator High correlated known poor prognosis, negative hormone receptor status, presence Her2, high-grade tumors, later stage. At same time, no strong correlation between survival was when all samples were analyzed together. Clear trend toward longer or seen several subgroups patients, most importantly significant association shorter disease-free early-stage low-grade BrCa, category highest demand predictive progression.