Sodium-specific membrane channels of frog skin are pores: current fluctuations reveal high turnover

作者: B Lindemann , W Van Driessche

DOI: 10.1126/SCIENCE.299785

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摘要: The reversible sodium transport blocker amiloride causes current fluctuations at the apical membrane of outer stratum granulosum frog skin. Their power density spectra reveal that single sites translocate more than 10(6) ions per second, which indicates a pore mechanism. open plus amiloride-blocked pores is in order 10(8) square centimeter skin area with 60 millimolar and 18 micromolar solution.

参考文章(10)
Gordon G. Hammes, Relaxation spectrometry of biological systems. Advances in Protein Chemistry. ,vol. 23, pp. 1- 57 ,(1968) , 10.1016/S0065-3233(08)60399-X
P. Lauger, Carrier-mediated ion transport. Science. ,vol. 178, pp. 24- 30 ,(1972) , 10.1126/SCIENCE.178.4056.24
Effects of vasopressin and aldosterone on amiloride binding in toad bladder epithelial cells Proceedings of The Royal Society B: Biological Sciences. ,vol. 189, pp. 543- 575 ,(1975) , 10.1098/RSPB.1975.0072
C.M. Armstrong, Evidence for ionic pores in excitable membranes Biophysical Journal. ,vol. 15, pp. 932- 933 ,(1975) , 10.1016/S0006-3495(75)85868-1
A. W. Cuthbert, W. K. Shum, Amiloride and the sodium channel. Naunyn-schmiedebergs Archives of Pharmacology. ,vol. 281, pp. 261- 269 ,(1974) , 10.1007/BF00500595
A. W. Cuthbert, An upper limit to the number of sodium channels in frog skin epithelium The Journal of Physiology. ,vol. 228, pp. 681- 692 ,(1973) , 10.1113/JPHYSIOL.1973.SP010106
PETER MITCHELL, A general theory of membrane transport from studies of bacteria. Nature. ,vol. 180, pp. 134- 136 ,(1957) , 10.1038/180134A0
Edward N. Ehrlich, Jean Crabb�, Jacqueline Scarlata, The mechanism of action of amipramizide. Pflügers Archiv: European Journal of Physiology. ,vol. 302, pp. 79- 96 ,(1968) , 10.1007/BF00586783
Thomas U. L. Biber, Molly L. Sanders, Influence of transepithelial potential difference on the sodium uptake at the outer surface of the isolated frog skin. The Journal of General Physiology. ,vol. 61, pp. 529- 551 ,(1973) , 10.1085/JGP.61.5.529
Bertil Hille, The Permeability of the Sodium Channel to Organic Cations in Myelinated Nerve The Journal of General Physiology. ,vol. 58, pp. 599- 619 ,(1971) , 10.1085/JGP.58.6.599