Function of a truncated dihydropyridine receptor as both voltage sensor and calcium channel

摘要: THE skeletal muscle dihydropyridine (DHP) receptor serves dual functions, as a voltage sensor for excitation–contraction coupling and an L-type calcium channel1–3. Biochemical analysis indicates the presence of two forms DHP polypeptide in muscle, full-length translation product present minor species much more abundant form that has truncated carboxy-terminus4–6. On basis these other observations7, it been proposed8 that, only can function channel coupling. To resolve this issue, we have now constructed complementary DNA (pC6Δl) encoding protein corresponding to muscle. Expression pC6Δl dysgenic myotubes fully restores both current, consistent with idea single class receptors performs functions.