NGS-based multiplex assay of trait-linked molecular markers revealed the genetic diversity of Iranian bread wheat landraces and cultivars

摘要: NGS-based multiplex assay has accomplished a valuable status as means of high throughput research, rapid screening functional markers in wheat breeding programs. Accordingly, we applied total 42 locus-specific from Indel and SNP-mediated categorisations coupled with the agronomic important genes or quantitative trait loci (QTL) bread wheat. The amplicons were analysed by an Ion Torrent Proton Sequencer. Then, allele detection custom pipeline was to process genotype 99 Iranian cultivars 270 landraces. On whole, 29 positively incorporated achieved 100% SNP call rates. Assessment sequence-tagged site (STS) competitive allele-specific PCR (KASP) concerning same confirmed calls all altogether. It revealed that landraces supply rich genetic resource capable resisting Hessian fly, leaf rust, fusarium head blight, adult plant diseases, stem soilborne mosaic virus, streak mosaic, pre-harvest sprouting, grain protein, gluten strength traits. This finding can be developed improve enrich Further, it is advocated promising approach for examining trait-linked germplasm collections.