Resonance Raman studies of isotopically labeled chloroperoxidase
作者： O. Bangcharoenpaurpong , P. M. Champion , K. S. Hall , L. P. Hager
DOI: 10.1021/BI00357A011
关键词:
摘要: Chloroperoxidase (CPO) and cytochrome P450cam have been shown by several techniques to similar active site properties. Recent resonance Raman investigations using isotopically enriched 34S-labeled samples demonstrated thiolate ligation in the system. We report here on a number of parallel studies involving CPO. On basis isotopic labeling (34S, 13CO), we assign Fe-S Fe-CO stretching frequencies CPO at 347 (-vFe-S) 488 cm-1 (-vFe-CO). The differences -vFe-S -vFe-CO may suggest subtle binding two systems.
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