Production of slime polysaccharides by Shigella dysenteriae type 1.

作者: Firdausi Qadri , Md Azizul Haque , Anwar Hossain , Manuel John Albert , None

DOI: 10.1111/J.1348-0421.1994.TB01738.X

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摘要: Electron microscopy of ruthenium red-stained ultrathin section strains Shigella dysenteriae type 1 grown in the Casamino Acids-yeast extract broth medium showed presence an extracellular slime layer. The appeared as a dense sheath covering bacteria. promoted hemagglutinating activity polysaccharide (SPS) isolated from cell-free culture supernatant or bacterial surface was less than 162,000 daltons size and immunochemically similar. SPS cross-reaction with lipopolysaccharide (LPS) antigen immunological tests; however, it also to be different LPS since did not contain 2-keto-3-deoxyoctonate, core sugar LPS. A pattern separation observed by silver staining SDS-polyacrylamide gels. From these data that either are contaminated each other is portion

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