Apparatus and method for mixed-bed lectin chromatography

摘要: The present invention contemplates an apparatus and method for isolating glycoconjugates from mixtures or contaminated thereof. In the invention, a bed other support element comprising mixture of immobilized lectins is provided. passed over mixed lectin wherein which correspond to in are bound thereto while non-glycoconjugates flow away. Thus, can be used isolate glycoproteins non-glycosylated proteins, glycopeptides peptides, glycolipids lipids, free oligosaccharides extracts preparations. This solves problem complex with non-gyconconjugates. For example, most cells, large fraction total macromolecules not glycosylated. Glycomics glycoproteomics specifically concerned contain carbohydrates. chromatography described herein will expand both glycomics glycoproteomics, currently hampered by lack methods devices approaches able generally all cells simple direct approach that has few steps.