Assembly of hundreds of novel bacterial genomes from the chicken caecum

作者: Laura Glendinning , Robert D. Stewart , Mark J. Pallen , Kellie A. Watson , Mick Watson

DOI: 10.1101/699843

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摘要: Abstract Chickens are a highly important source of protein for large proportion the human population. The cecal microbiota plays crucial role in chicken nutrition through production short chain fatty acids, nitrogen recycling and amino acid production. In this study we sequenced DNA from contents samples taken 24 chickens belonging to either fast or slower growing breed consuming vegetable-only diet containing fish meal. We utilised 1.6T Illumina data construct 469 draft metagenome-assembled bacterial genomes, including 460 novel strains, 283 species 42 genera. also compared abundance these carbohydrate active enzymes they produce, between our groups demonstrate that there both breed- diet- specific microbiomes, as well an overlapping core microbiome. This will form basis future studies examining composition function microbiota.

参考文章(104)
Jennifer Fouquier, Jai Ram Rideout, Evan Bolyen, John Chase, Arron Shiffer, Daniel McDonald, Rob Knight, J Gregory Caporaso, Scott T. Kelley, ghost-tree: creating hybrid-gene phylogenetic trees for diversity analyses Microbiome. ,vol. 4, pp. 11- 11 ,(2016) , 10.1186/S40168-016-0153-6
Jianhua Gong, Weiduo Si, Robert J Forster, Ruilin Huang, Hai Yu, Yulong Yin, Chengbo Yang, Yanming Han, None, 16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to ceca. FEMS Microbiology Ecology. ,vol. 59, pp. 147- 157 ,(2007) , 10.1111/J.1574-6941.2006.00193.X
Francesco Asnicar, George Weingart, Timothy L. Tickle, Curtis Huttenhower, Nicola Segata, Compact graphical representation of phylogenetic data and metadata with GraPhlAn PeerJ. ,vol. 3, ,(2015) , 10.7717/PEERJ.1029
Venessa Eeckhaut, Filip Van Immerseel, Siska Croubels, Siegrid De Baere, Freddy Haesebrouck, Richard Ducatelle, Petra Louis, Peter Vandamme, Butyrate production in phylogenetically diverse Firmicutes isolated from the chicken caecum Microbial Biotechnology. ,vol. 4, pp. 503- 512 ,(2011) , 10.1111/J.1751-7915.2010.00244.X
B. Yu, J.R. Liu, F.S. Hsiao, P.W.S. Chiou, Evaluation of Lactobacillus reuteri Pg4 strain expressing heterologous β-glucanase as a probiotic in poultry diets based on barley Animal Feed Science and Technology. ,vol. 141, pp. 82- 91 ,(2008) , 10.1016/J.ANIFEEDSCI.2007.04.010
Alexander Lex, Nils Gehlenborg, Hendrik Strobelt, Romain Vuillemot, Hanspeter Pfister, UpSet: Visualization of Intersecting Sets. IEEE Transactions on Visualization and Computer Graphics. ,vol. 20, pp. 1983- 1992 ,(2014) , 10.1109/TVCG.2014.2346248
V. Iverson, R. M. Morris, C. D. Frazar, C. T. Berthiaume, R. L. Morales, E. V. Armbrust, Untangling genomes from metagenomes: Revealing an uncultured class of marine euryarchaeota Science. ,vol. 335, pp. 587- 590 ,(2012) , 10.1126/SCIENCE.1212665
D Józefiak, A Rutkowski, S.A Martin, Carbohydrate fermentation in the avian ceca: a review Animal Feed Science and Technology. ,vol. 113, pp. 1- 15 ,(2004) , 10.1016/J.ANIFEEDSCI.2003.09.007