Biomaterials Design for Control of Cell Behavior by Femtosecond Laser Processing

摘要: The last decade has seen exciting and unprecedented work at the interface between biology materials science, particularly in form of exquisite control cell attachment, shape, traction, motility, differentiation. Recent progress developing techniques for microfabrication biomaterials helps recapitulate many extracellular matrix (ECM) cues, making them progressively more useful applications tissue engineering. This dissertation presents a study femtosecond laser assisted micro- nano-fabrication applicable design aiming achieving deliberate behavior. Cell mechanics connected to alignment migration, from speculation response surface by topographic chemical patterns, was studied. migration is an essential cellular process variety physiological pathological phenomena. Migration leukocytes mediates phagocytic immune responses. fibroblasts, vascular endothelial cells, osteoblasts contributes wound healing regeneration, tumor metastasis. can be initiated mechanical cues microenvironment. Factors affecting both soluble insoluble macromolecules that comprise ECM or mediate communication. We apply induced two-photon polymerization multiphoton ablation lithography fabricate precisely defined two-dimensional patterned nanometer micrometer length scale three-dimensional filamentous used studies addressing fundamental issues concerning adhesion migration. studied microscale topographical Anisotropic micronscale ridge/groove surfaces are powerful shape enhance obstruct However, they have limited ability independently size distribution adhesive domains ligand density. Thus, we applied chemically topographically nanoscale guide directional overcome constraints patterns. During contractile force needed move body forward. also exerted individual locomoting using fiber scaffolds.