High molecular weight DNA assembly in vivo for synthetic biology applications
作者： Mario Juhas , James W. Ajioka
DOI: 10.3109/07388551.2016.1141394
关键词:
摘要: DNA assembly is the key technology of emerging interdisciplinary field synthetic biology. While smaller fragments usually performed in vitro, high molecular weight molecules are assembled vivo via homologous recombination host cell. Escherichia coli, Bacillus subtilis and Saccharomyces cerevisiae main hosts used for vivo. Progress over last few years has paved way construction whole genomes. This review provides an update on recent biology advances with particular emphasis E. B. S. cerevisiae. Special attention paid to genomes, such as those first cell, yeast minimal
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