Meiotic progression of isolated mouse spermatocytes under simulated microgravity.

摘要: Progression through the prophase of first meiotic division can be obtained in culture by treatment mouse spermatocytes with serine/threonine phosphatase inhibitor okadaic acid. Chromosome condensation during this G2/M transition involves activation MAPK pathway, which causes Nek2 and phosphorylation chromatin architectural protein Hmga2. In an effort to set up conditions allow a spontaneous progression meiosis, we have investigated cell-cycle features these cells cultured for 24 h rotary cell system humidified atmosphere thermostatic incubator simulate microgravity environment. Morphological analysis nuclear squashes indicated 2-fold increase late-pachytene highly condensed chromosomes, contemporaneous decrease mid-pachytene less chromatin. Microgravity induced cyclinB-cdc2 complex, confirming at molecular level that had occurred. Moreover, using immuno-kinase assays specific substrates demonstrated under is accompanied Erk1/p90Rsk2 pathway. These data pathway correlates even occurs spontaneously not drug. We suggest might help release block inhibits isolated from progressing unit gravity, study physiological events germ differentiation vitro.