Scribble mutation disrupts convergent extension and apical constriction during mammalian neural tube closure

摘要: Abstract Morphogenesis of the vertebrate neural tube occurs by elongation and bending plate, tissue shape changes that are driven at cellular level polarized cell intercalation changes, notably apical constriction wedging. Coordinated intercalation, constriction, wedging undoubtedly require complex underlying cytoskeletal dynamics remodeling adhesions. Mutations gene encoding Scribble result in defects mice, however molecular mechanisms which Scrib regulates behavior remain unknown. Analysis mutants revealed live imaging mouse embryos showed mutation results particularly rosette resolution, failure both mutant displayed aberrant expression junctional proteins ZO-1, Par3, Par6, E- N-cadherins, actin myosin. These findings show has a central role organizing complexes regulating morphomechanical behaviors neurulation, they advance our understanding involved mammalian closure. Highlights Polarized is lost specific effects on formation resolution disrupts necessary for closure Adherens tight junction composition altered epithelial cells