Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum
作者： Nicolas Feau , Tod D. Ramsfield , Renate Heinzelmann , Rebecca McDougal , Richard C. Hamelin
DOI: 10.3390/F12030362
关键词:
摘要: A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity LAMP tested using a selection needle fungi, including pini Hulbary, and Lecanosticta acicola (Thum.) Syd.; only D. DNA amplified by test. In terms sensitivity, able to detect as little 1 pg total DNA. This enables extracted from diseased host needles be rapidly presence relatively simple operate equipment away fully equipped molecular biology laboratory.
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