Fabrication of an EGF modified nanodiamonds-based anti-cancer drug targeted delivery system and drug carrier uptake visualization by 3D Raman microscopy

摘要: A nanodiamonds-based anti-cancer drug targeted delivery system, Epidermal Growth Factor (EGF)–nanodiamonds (NDs)–cisplatin (ENC) bioconjugate was developed. Due to the effect of specific biorecognition process EGF (a kind targeting molecule), human liver hepatocellular carcinoma (HepG2) cells could be selectively killed by ENC even in a lower concentration cisplatin drug) comparing traditional therapy. The cytotoxicity blocked EGFR antibody cancer cells, revealing specificity its ability. Because morphological change HepG2 treated observed, from epithelial mesenchymal-like shape, related mechanism evaluated. results showed that E-cadherin expression declined after treatment, while vimentin did not change. decrease means loss cell–cell adhesion. Loose provided more surface area for absorption. Based on these advantages, system would promising system. Moreover, we located spatial distribution using NDs existing as Raman probe through 3-dimensional (3D) confocal imaging, i.e., were able visualize uptake into living which is helpful observe between and receptor (EGFR) physiology condition cellular present study demonstrates drug-loaded have potential novel intravascular probes both diagnostic (e.g., imaging) therapeutic purposes delivery).