Functional analysis of NtMPK2 uncovers its positive role in response to Pseudomonas syringae pv. tomato DC3000 in tobacco

作者: Xingtan Zhang , Genhong Wang , Junping Gao , Mengyun Nie , Wenshan Liu

DOI: 10.1007/S11103-015-0391-1

关键词:

摘要: Mitogen-activated protein kinase cascades are highly conserved signaling modules downstream of receptors/sensors and play pivotal roles in plant defense against pathogen attack. Extensive studies on Arabidopsis MPK4 have implicated that the MAP is involved multilayered pathways. In this study, we identified tobacco NtMPK2 as an ortholog AtMPK4. Transgenic overexpressing markedly enhances resistance to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) virulent avirulent strains. Transcriptome analysis NtMPK2-dependent genes shows possibly basal system activated by overexpression. addition NtMPK2-mediated resistance, multiple pathways response bacteria based Pst-responding genes, including SA ET Notably, it possible biosynthesis antibacterial compounds responsible for inhibition Pst strain when programmed cell death processes host. Our results uncover positively regulate improve our understanding molecular mechanism.

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