The selenium requirement for glutathione peroxidase mRNA level is half of the selenium requirement for glutathione peroxidase activity in female rats.

摘要: To determine critically the selenium (Se) requirement for weanling female rats, we used glutathione peroxidase (GSH: H2O2 oxidoreductase, EC 1.11.1.9) (GPX) mRNA and a number of other parameters to assess Se status. Rats were fed basal torulayeast diet (0.007 micrograms Se/g) supplemented with as Na2SeO3 in graded levels from 0 0.3 Se/g 32 d (3 rats/group). Selenium supplementation had no effect on growth, showing that growth is less than 0.007 diet, whereas showed significant increases supplementation. In rats Se-deficient liver concentration was 4 +/- 0%, plasma GPX activity 8 1%, erythrocyte 40 3%, 2 11-17% 0.1 diet. Liver plasma, erythrocytes all reached plateau breakpoint at or near indicating dietary maximal growing 0.05 minimum half activity. This experiment demonstrates can be requirement; gap between necessary may represent an evolutionarily derived biological margin safety.