Proteomic analysis of normal and malignant prostate tissue to identify novel proteins lost in cancer

摘要: BACKGROUND. Alterations of important protein pathways, including loss prostate secretory granules, and disruption the prostatic pathway have been identified as early events in malignancy. In this study, proteomics was used to map differences expression between normal malignant tissues identify analyze differentially expressed proteins human tissue with particular regard lost METHODS. Small quantities were taken fresh from 34 radical prostatectomy cases. After histological examination, solubilized selected separated using two-dimensional electrophoresis. Using image analysis, proteome mapped (present absent tissue) subsequently analyzed peptide mass finger printing N-terminal sequencing. Western blotting immunohistochemistry performed examine profiles localization candidate proteins. RESULTS. Comparison maps 20 which transformation, specific antigen (PSA), alpha-l antichymotrypsin (ACT), haptoglobin, lactoylglutathione lyase. Three had not previously reported (Ubiquitin-like NEDD8, calponin, a follistatin-related protein). confirmed NEDD8 demonstrated cellular these two glands. CONCLUSIONS. The is novel finding role functional their or reduced malignancy warrants further investigations. (C) 2002 Wiley-Liss, Inc.