Nitric oxide synthase and changes in oxidative stress levels in embryonic kidney observed in a rabbit model of intrauterine growth restriction.
作者： Horacio Figueroa , Jorge Cifuentes , Mauricio Lozano , Cristobal Alvarado , Claudia Cabezas
DOI: 10.1002/PD.4829
关键词:
摘要: OBJECTIVE This work aimed to study the effect of uteroplacental circulation restriction on endothelial kidney damage in a fetal rabbit model. METHODS New Zealand rabbits were subjected 40% 50% artery ligation at day 25 pregnancy. After 5 days, surviving fetuses harvested by cesarean section. The gene and protein expressions selected enzymes associated with nitric oxide production oxidative stress analyzed homogenates. RESULTS placenta weight (6.06 ± 0.27, p < 0.0319) body (19.90 ± 1.03, p < 0.0001) significantly reduced group. kidneys from restricted presented mild vascular congestion glomerular capillary congestion, without inflammation or hypertrophy. We found synthase phosphorylation inhibition (0.23 ± 0.13, p < 0.012) arginase-2 (0.29 ± 0.14, p < 0.023) induction Finally, circulation-restricted showed increased inducible messenger RNA (mRNA) (2.68 ± 0.24, p < 0.01) heme oxygenase-1 mRNA (23 ± 1.3, p < 0.003), reactive oxygen species (1.69 ± 0.09, p < 0.001) nitrotyrosine (1.74 ± 0.28, p < 0.003) levels, changes Nox mRNA. CONCLUSION describe significant deregulation activity that have been exposed uterine circulation. © 2016 John Wiley & Sons, Ltd.
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